Isolated Organ Experiments with the rat uterus
It was part of my thesis and I was doing an experiment on an isolated organ, rat uterus. Depolarizing the tissue with potassium sulfate in a calcium-free medium, a flaccid organ was being obtained, not to contract anymore, even with the addition of acethylcholine had no effect. It was a labourious process. I had to dissect rat uterus, depolarize it with potassium sulfate and wait until it become totally relaxed. In this stage, only the addition of calcium was able to induce tonic contractions. Concentration of calcium needed to induce contractions was in the millimolar range.
Wedding ceremony - All of us were there!
The Great Staff! For the memory of now deceased friend,